: The fragile plantlets are gradually transitioned from the high-humidity sterile environment to soil and natural light conditions. Essential Requirements
: Individual shoots are transferred to a rooting medium, usually containing auxins (like IBA or NAA), to develop a healthy root system. Plant Propagation by Tissue Culture
To succeed, tissue culture requires a highly controlled environment: : The fragile plantlets are gradually transitioned from
: The explant is moved to a medium enriched with hormones (often cytokinins like BAP) to stimulate the growth of multiple new shoots. Plant Propagation by Tissue Culture
: A small piece of plant tissue (the explant ), such as a shoot tip or bud, is sterilized and placed on an initial growth medium.